Determination of Thujone by Derivatization with Dansylhydrazine and Liquid Chromatography.

The reaction of α- and β-thujones with dansylhydrazine (DNSH) was studied using reversed phase liquid chromatography (LC) with fluorescence detection. The derivatization procedure, although slow, was applied for determination of α-thujone in white vermouth at concentrations 0.45–4.5 µg/L using a C18 solid phase extraction column for cleanup; recoveries of added α-thujone were in the range 60–77%. Separation of dansyl α- and β-thujones was achieved by LC and their identity confirmed by LC-tandem mass spectrometry (MS/MS). [ABSTRACT FROM AUTHOR]