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Layer-by-layer deposition of hyaluronic acid and poly-l-lysine for patterned cell co-cultures
- Source :
-
Biomaterials . Aug2004, Vol. 25 Issue 17, p3583. 10p. - Publication Year :
- 2004
-
Abstract
- A novel method for patterning cellular co-cultures that uses the layer-by-layer deposition of ionic biopolymers is described. Non-biofouling hyaluronic acid (HA) micropatterns were used to immobilize cells and proteins to glass substrates. Subsequent ionic adsorption of poly-l-lysine (PLL) to HA patterns was used to switch the HA surfaces from cell repulsive to adherent thereby facilitating the adhesion of a second cell type. The utility of this approach to pattern co-cultures of hepatocytes or embryonic stem cells with fibroblasts was demonstrated. In addition, the versatility of this approach to generate patterned co-cultures irrespective of the primary cell seeding and relative adhesion of the seeded cells was demonstrated. Thus, the proposed method may be a useful tool for fabricating controlled cellular co-cultures for cell–cell interaction studies and tissue engineering applications. [Copyright &y& Elsevier]
- Subjects :
- *HYALURONIC acid
*LIVER cells
*CELL communication
*BIOPOLYMERS
Subjects
Details
- Language :
- English
- ISSN :
- 01429612
- Volume :
- 25
- Issue :
- 17
- Database :
- Academic Search Index
- Journal :
- Biomaterials
- Publication Type :
- Academic Journal
- Accession number :
- 12501122
- Full Text :
- https://doi.org/10.1016/j.biomaterials.2003.10.033