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Role of Gut Inflammation in Altering the Monocyte Compartment and Its Osteoclastogenic Potential in HLA-B27-Transgenic Rats.
- Source :
-
Arthritis & Rheumatology . Sep2017, Vol. 69 Issue 9, p1807-1815. 9p. - Publication Year :
- 2017
-
Abstract
- Objective To investigate the relationship between intestinal inflammation and the central and peripheral innate immune system in the pathogenesis of HLA-B27-associated spondyloarthritis using an HLA-B27-transgenic (B27-Tg) rat model. Methods The myeloid compartment of the blood and bone marrow (BM) of B27-Tg rats, as well as HLA-B7-Tg and non-Tg rats as controls, was evaluated by flow cytometry. Plasma from rats was assessed by enzyme-linked immunosorbent assay for levels of CCL2 and interleukin-1α (IL-1α). Rats were treated with antibiotics for 4 weeks, and the myeloid compartment of the blood and BM was evaluated by flow cytometry. The osteoclastogenic potential of BM-derived cells from antibiotic-treated rats, in the presence or absence of tumor necrosis factor (TNF), was evaluated in vitro. Results B27-Tg rats had substantially higher numbers of circulating Lin−CD172a+CD43low monocytes as compared to control animals, and this was significantly correlated with higher levels of plasma CCL2. Antibiotic treatment of B27-Tg rats markedly reduced the severity of ileitis, plasma levels of CCL2 and IL-1α, and number of BM and blood Lin−CD172a+CD43low monocytes, a cell subset shown in the present study to have the greatest in vitro osteoclastogenic potential. Antibiotic treatment also prevented the TNF-dependent enhancement of osteoclastogenesis in B27-Tg rats. Conclusion Microbiota-dependent intestinal inflammation in B27-Tg rats directly drives the systemic inflammatory and bone-erosive potential of the monocyte compartment. [ABSTRACT FROM AUTHOR]
- Subjects :
- *ILEITIS
*ANIMAL experimentation
*ANKYLOSING spondylitis
*ANTIBIOTICS
*BIOLOGICAL models
*ENZYME-linked immunosorbent assay
*FLOW cytometry
*INFLAMMATION
*INTERLEUKINS
*INTESTINES
*MONOCYTES
*RATS
*TUMOR necrosis factors
*GUT microbiome
*STATISTICAL significance
*IN vitro studies
*IN vivo studies
*PREVENTION
IMMUNE system physiology
Subjects
Details
- Language :
- English
- ISSN :
- 23265191
- Volume :
- 69
- Issue :
- 9
- Database :
- Academic Search Index
- Journal :
- Arthritis & Rheumatology
- Publication Type :
- Academic Journal
- Accession number :
- 124865956
- Full Text :
- https://doi.org/10.1002/art.40154