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Development of a LC–MS/MS method for the determination of CKD-712 in rat plasma: Application to a pharmacokinetic study in rats.
- Source :
-
Journal of Chromatography B: Analytical Technologies in the Biomedical & Life Sciences . Sep2017, Vol. 1061, p123-127. 5p. - Publication Year :
- 2017
-
Abstract
- CKD-712 is a potential treatment for sepsis, as it exhibits protective effects against lipopolysaccharide-mediated platelet aggregation, inducible nitric oxide synthase expression, and cecum-ligation puncture-induced septic mortality in mice. In this study, we develop a rapid and sensitive LC–MS/MS method for determining CKD-712 in rat plasma. CKD-712 and papaverine hydrochloride (an internal standard) were analyzed using an LC–MS/MS system consisting of an Agilent HPLC system (HP-1100) equipped with an Atlantis HILIC Silica (2.1 × 50 mm, 3 μm) column and a API 4000 (Applied Biosystems/MDS Sciex, USA) in a positive ESI mode. We utilized multiple reaction monitoring (MRM) at m / z transitions of 306.2–164.0 to analyze CKD-712, and 340.3–202.1 m / z for IS, with a mobile phase of acetonitrile (0.025% trifluoroacetic acid):20 mM ammonium acetate (94:6, v/v) at a flow rate of 0.25 mL/min. The lower limit of quantification (LLOQ) was 5 ng/mL, with a linearity ranging from 5 to 1000 ng/mL ( r > 0.999). Validation parameters including specificity, precision, accuracy, matrix effect, recovery, dilution effect and stability results were well within acceptance criteria, and applied successfully on a pharmacokinetic study in rats. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 15700232
- Volume :
- 1061
- Database :
- Academic Search Index
- Journal :
- Journal of Chromatography B: Analytical Technologies in the Biomedical & Life Sciences
- Publication Type :
- Academic Journal
- Accession number :
- 124822753
- Full Text :
- https://doi.org/10.1016/j.jchromb.2017.07.011