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Lipocalin-Type Prostaglandin D Synthase in Urine in Adriamycin-Induced Nephropathy of Mice.
- Source :
-
Nephron Physiology . 2004, Vol. 96 Issue 2, pp42-p51. 1p. - Publication Year :
- 2004
-
Abstract
- Background/Aims: Lipocalin-type prostaglandin D synthase (L-PGDS), an enzyme converting prostaglandin H[sub 2] to prostaglandin D[sub 2] , occurs particularly in the cardiovascular system. Urinary L-PGDS excretion is increased in diabetes prior to overt proteinuria, suggesting that it is a predictor of renal injury. In this study, we tested the hypothesis that L-PGDS excretion reflects renal injury in primary glomerular diseases using Adriamycin-induced nephropathy in mice. Methods: Twenty 6-week-old ICR female mice were intravenously given a dose of 25 mg Adriamycin/kg body weight through the tail vein. 24-hour urine was collected every day, and blood samples were obtained. Results: The mice developed significant albuminuria from day 3 onward (p < 0.05), which was followed by overt proteinuria from day 4 (p < 0.05). Histological examination revealed focal mesangial expansion with partial tubular atrophy. Urinary L-PGDS excretion significantly increased from day 1 onward (p < 0.05), and apparently preceded the increase in urinary albumin excretions. Either serum L-PGDS or creatinine levels were not changed by administration of Adriamycin. However, serum creatinine levels were inversely correlated to urinary L-PGDS excretions (r = –0.88, p < 0.05). Immunohistochemistry showed that L-PGDS occurred in the tubules, but not in the glomeruli in Adriamycin mice and L-PGDS mRNA paralleled urinary L-PGDS excretion. Conclusion: Urinary L-PGDS excretion is increased in Adriamycin-induced nephropathy, and this precedes overt albuminuria. Copyright © 2004 S. Karger AG, Basel [ABSTRACT FROM AUTHOR]
- Subjects :
- *ENZYMES
*PROSTAGLANDINS
*URINE
*DOXORUBICIN
*KIDNEY diseases
*MICE
Subjects
Details
- Language :
- English
- ISSN :
- 16602137
- Volume :
- 96
- Issue :
- 2
- Database :
- Academic Search Index
- Journal :
- Nephron Physiology
- Publication Type :
- Academic Journal
- Accession number :
- 12473150
- Full Text :
- https://doi.org/10.1159/000076407