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Biofunctional Polymer-Lipid Hybrid High-Density Lipoprotein-Mimicking Nanoparticles Loading Anti-miR155 for Combined Antiatherogenic Effects on Macrophages.
- Source :
-
Biomacromolecules . Aug2017, Vol. 18 Issue 8, p2286-2295. 10p. - Publication Year :
- 2017
-
Abstract
- A biofunctional polymer-lipid hybrid high-density lipoprotein-mimicking nanoparticle (HNP) loading anti-miR155 was constructed for combined antiatherogenic effects on macrophages. The HNP consisted of an anti-miR155 core condensed by acid-labile polyethylenimine (acid-labile PEI) polymers and a lipid bilayer coat that was decorated with apolipoprotein A-1, termed acid-labile PEI/HNP. The acid-labile PEI was synthesized with low-molecular-weight PEI and glutaraldehyde to reduce the cytotoxicity and facilitate nucleic acids escaping from acidic endolysosomes. The increased silencing efficiency of acid-labile PEI/HNP was ascribed to the clathrin-mediated endocytosis and successful endolysosomal escape. Decreased intracellular reactive oxygen species production and DiI-oxLDL uptake revealed the antioxidant activities of both anti-miR155 and HNP. Cholesterol efflux assay indicated the potential of HNP in reverse cholesterol transport. Collectively, the acid-labile PEI/HNP not only realized the efficacy of anti-miR155 in macrophages but also exerted the antiatherosclerotic biofunction of HNP. [ABSTRACT FROM AUTHOR]
- Subjects :
- *MACROPHAGES
*APOLIPOPROTEIN A
*GLUTARALDEHYDE
*ENDOCYTOSIS
*POLYMERS
Subjects
Details
- Language :
- English
- ISSN :
- 15257797
- Volume :
- 18
- Issue :
- 8
- Database :
- Academic Search Index
- Journal :
- Biomacromolecules
- Publication Type :
- Academic Journal
- Accession number :
- 124640796
- Full Text :
- https://doi.org/10.1021/acs.biomac.7b00436