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Optimization, purification and structural characterization of a dextran produced by L. mesenteroides isolated from Chinese sauerkraut.
- Source :
-
Carbohydrate Polymers . Oct2017, Vol. 174, p409-416. 8p. - Publication Year :
- 2017
-
Abstract
- A higher yield of dextran strain Leuconstoc mesenteroides TDS2-19 was isolated from Chinese sauerkraut juice. Effects of the three main factors on exopolysaccharide (EPS) yield were investigated by central composite design (CCD) and the optimum composition was sucrose 117.48 g/L, sodium acetate 4.10 g/L, and initial pH 6.88. Optimum results showed that EPS yield was increased to 71.23 ± 2.25 g/L in 48 h fermentation, 31.24% higher than before. The molecular weight (Mw) of ESP was 8.79 × 10 7 Da, as determined by high-performance size-exclusion chromatography (HPSEC). Fourier transform infrared spectra (FT-IR) and nuclear magnetic resonance spectra (NMR) showed that the polysaccharide synthesized by L. mesenteroides TDS2-19 in the MRS medium was dextran with a peak, a linear backbone composed of consecutive α-(1 → 6)-linked d -glucopyranose units. No branching was observed in the dextran structure. The present study suggested that L. mesenteroides TDS2-19 might be used for the industrial-scale production of linear dextran. [ABSTRACT FROM AUTHOR]
- Subjects :
- *DEXTRAN
*SAUERKRAUT
*MICROBIAL exopolysaccharides
*SUCROSE
*SODIUM acetate
Subjects
Details
- Language :
- English
- ISSN :
- 01448617
- Volume :
- 174
- Database :
- Academic Search Index
- Journal :
- Carbohydrate Polymers
- Publication Type :
- Academic Journal
- Accession number :
- 124630525
- Full Text :
- https://doi.org/10.1016/j.carbpol.2017.06.084