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Purification and Properties of a Polynucleotide Ligase from Human Cell Cultures.
- Source :
-
European Journal of Biochemistry . 1971, Vol. 22 Issue 1, p75-78. 4p. - Publication Year :
- 1971
-
Abstract
- A polynucleotide ligase has been detected and purified over 300-fold from human cells cultured in vitro. The enzymic activity is dependent on ATP and Mg2+. The pH curve is bimodal with two optima at pH 7.5 and 8.1. The purified enzyme requires for its activity a heat-resistant protein factor present in the crude extract. [ABSTRACT FROM AUTHOR]
- Subjects :
- *NUCLEIC acids
*CELL culture
*ENZYMES
*PROTEINS
Subjects
Details
- Language :
- English
- ISSN :
- 00142956
- Volume :
- 22
- Issue :
- 1
- Database :
- Academic Search Index
- Journal :
- European Journal of Biochemistry
- Publication Type :
- Academic Journal
- Accession number :
- 12455528
- Full Text :
- https://doi.org/10.1111/j.1432-1033.1971.tb01516.x