月季和野蔷薇的试管嫁接研究.

【Objective】 Study on the technology of in vitro grafting of Rosa chinensis and Rosa multiflora Thunb. Fl. Jap provided effective ways for the propagation of rose cutting. 【Method】 With the Rosa multifora Thunb. Fl. Jap. bud stem segments as explants，by comparing different sterilization methods，different types and concentrations of hormone medium influence on tissue micropropagation，screened out suitable sterilization method and culture medium，thus established sterile rapid breeding system of the Rosa multiflora Thunb. Fl. Jap.，and in different cultivars of sterile Rosa chinensis's seedling as a scion，the Rosa multijlora Thunb. Fl. Jap. was conducted on distant related grafting for the root stock. 【Result】 The results showed that the Rosa multiflora Thunb. Fl. Jap. was suitable for sterilization of explants with 75 % alcohol solution to wipe the surface of the stems after soaking for 10 seconds，and soaks with 0. 1 % mercuric chloride solution for 10 minutes，subculture proliferating medium was MS + 6-BA 3. 0 mg/L + 0.10 mg/L NAA，the rooting medium was WPM n NAA 0. 05 mg/L. Keeping the root stock blade was helpful to enhance the survival rate of grafting and rooting rate ； It was beneficial to the survival of distant grafting by the subculture proliferating medium of 25 d of Rosa chinensis stem tip as a scion. The highest survival rate of transplanting was Hiohgi-multiflora，followed by Carola-Rosa multiflora Thunb. Fl. Jap.， Golden Scepter-Roa multiflora Thunb. Fl. Jap.，Double Delight- Rosa multiflora Thunb. Fl. Jap.， Rose Eden-Rosa multiflora Thunb. Fl. Jap.， and Heritage-Roa multiflora Thunb. Fl. Jap. 【Conclusion 】 The survival rate of different varieties of Rosa chinensis and Rosa multiflora Thunb. Fl. Jap. was different with variety. There was close relationship between rootstock retaining leaves or not ，the subculture time and grafting survival rate. [ABSTRACT FROM AUTHOR]