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Selective depletion of cultured macrophages by magnetite nanoparticles modified with gelatin.
- Source :
-
Experimental & Therapeutic Medicine . Aug2017, Vol. 14 Issue 2, p1640-1646. 7p. - Publication Year :
- 2017
-
Abstract
- Previous studies have indicated pro-tumor functions of macrophages in tumor progression in different types of malignant tumors. The detailed mechanisms of cell-cell interaction between macrophages and tumor cells have been investigated by means of in vitro co-culture experiments. The present study developed magnetite nanoparticles modified with gelatin that are specifically engulfed by macrophages and investigated methods to deplete these macrophages in co-culture experiments using a magnet. T98G glioma cell line and human monocyte-derived macrophages were mixed and co-cultured for 2 days. The T98G cells were isolated by depletion of the macrophages using the magnetite nanoparticles. mRNA expression of a number of pro-tumor molecules in the isolated T98G cells, with or without co-culture with macrophages, was then evaluated. The mRNA expression levels of chemokine (CC motif) ligand 2, interleukin-6 and macrophage-colony stimulating factor receptor (M-CSFR) were significantly upregulated in T98G cells by co-culture with macrophages (P<0.01). M-CSFR protein expression was also increased by co-culture with macrophages. The conditioned medium of co-cultured cells increased M-CSFR expression in T98G cells. Magnetite nanoparticles may be a novel tool not only for investigating the unique activation status of tumor cells in co-culture conditions, but also for targeting pro-tumor macrophages in tumor tissues. [ABSTRACT FROM AUTHOR]
- Subjects :
- *NANOPARTICLES
*MAGNETITE
*MACROPHAGES
*GELATIN
*CANCER invasiveness
Subjects
Details
- Language :
- English
- ISSN :
- 17920981
- Volume :
- 14
- Issue :
- 2
- Database :
- Academic Search Index
- Journal :
- Experimental & Therapeutic Medicine
- Publication Type :
- Academic Journal
- Accession number :
- 124347043
- Full Text :
- https://doi.org/10.3892/etm.2017.4640