地塞米松抑制烟曲霉菌暴露的支气管哮喘 大鼠肺组织IL-33的表达.

Objective To investigate the levels of IL-33 in the lung induced by Aspergillus fumigatus (A. fumigatus) exposure in bronchial asthmatic rats and the effects of dexamethasone. Methods Twenty-four Wistar rats were randomly and equally divided into group A (normal control), group B (asthma group)，group C (A. fumigtu-exposed group)，and group D (dexamethasone-treated group). B,C and D group were sensitized and challenged with ovalbumin (OVA) to establish asthmatic models. Then?group C and D were given A. fumigatus spores intranasally. Group D was pre-treated with dexamethasone during ovalbumin challenge. Group A was sensitized and challenged intranasally with normal saline as control. Airway hyperresponsiveness，eosinophils percentage and serum IgE levels were measured to confirm the establishment of asthmatic model. Expression of IL-33 in the lung were quantified by ELISA and qRT-PCR. Lung tissues and blood were plated on the potato dextrose agar (PDA) medium and cultivated for 24 h to measure the number of colony. Results Eosinophils percentage in bronchoalveolar lavage fluid (BALF) ?IgE levels in serum and IL-33 levels in the lung in group B and C were much more higher than in group A (P$0. 05) ?and the increasing range in group C was more obvious than group B. However?the changes of sRaw values in these groups had no statistical significance. Esosinophils percentage in BALF and IL-33 levels in the lung were significantly decreased (P$0. 05) ?and IgE levels in serum was slightly decreased (P%0.05) in group D when compared with group C ， but the ratio of A . fumigatus colonization in the lung was higher than group C. Conclusions Dexamethasone can inhibit the expression of IL-33 in the lung induced by A. exposure in an asthmatic rat?but it also increases the risk of fungal colonization in the airway. Anti-IL-33 antibody treatment needs further research. [ABSTRACT FROM AUTHOR]