转 Bt 基因抗虫棉外源 Bt 蛋白的差异表达.

【Objective】 Studying on the temporal and spatial dynamics of Bt protein expression in Bt transgenic cotton and determining the relation of Bt protein expression and Si transgenic cotton can effectively reduce the harm of target pests.【Method】 In this paper, the Bt protein contents in the different tissues, organs, main stem functional leaves and main stem leaves at different positions of transgenic cotton cultivar (GK19) in different growth stages were studied.【Result】The Bt protein contents of all tissues and organs were different, which were in order of seedling > flower > boll > bud > root > stem. There was a reduction tendency of Bt protein in main stem functional leaves in different growth stages with the advance of development. The Bt protein contents among main stem leaves at different positions in different growth stages were obviously different, which indicated the gradual decrease from the first to the seventh leaves in seedling and full budding stage and a gradual stabilization after a slow increase from the first to the seventh leaves in full flowering and boiling stages.【Conclusion】The Bt protein in all growth periods, all tissues and organs of Bt transgenic cotton were expressed, which changed with temporal and spatial dynamics. [ABSTRACT FROM AUTHOR]