Back to Search Start Over

BmajPLA2-II, a basic Lys49-phospholipase A2 homologue from Bothrops marajoensis snake venom with parasiticidal potential.

Authors :
Grabner, Amy N.
Alfonso, Jorge
Kayano, Anderson M.
Moreira-Dill, Leandro S.
Dos Santos, Ana Paula De A.
Caldeira, Cleópatra A.s.
Sobrinho, Juliana C.
Gómez, Ana
Grabner, Fernando P.
Cardoso, Fabio F.
Zuliani, Juliana Pavan
Fontes, Marcos R.m.
Pimenta, Daniel C.
Gómez, Celeste Vega
Teles, Carolina B.g.
Soares, Andreimar M.
Calderon, Leonardo A.
Source :
International Journal of Biological Macromolecules. Sep2017, Vol. 102, p571-581. 11p.
Publication Year :
2017

Abstract

Snake venoms contain various proteins, especially phospholipases A 2 (PLA 2 s), which present potential applications in diverse areas of health and medicine. In this study, a new basic PLA 2 from Bothrops marajoensis with parasiticidal activity was purified and characterized biochemically and biologically. B. marajoensis venom was fractionated through cation exchange followed by reverse phase chromatographies. The isolated toxin, BmajPLA 2 -II, was structurally characterized with MALDI-TOF (Matrix-assisted laser desorption/ionization-time of flight) mass spectrometry, sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), followed by two-dimensional electrophoresis, partial amino acid sequencing, an enzymatic activity assay, circular dichroism, and dynamic light scattering assays. These structural characterization tests presented BmajPLA 2 -II as a basic Lys49 PLA 2 homologue, compatible with other basic snake venom PLA 2 s (svPLA 2 ), with a tendency to form aggregations. The in vitro anti-parasitic potential of B. marajoensis venom and of BmajPLA 2 -II was evaluated against Leishmania infantum promastigotes and Trypanosoma cruzi epimastigotes, showing significant activity at a concentration of 100 μg/mL. The venom and BmajPLA 2 -II presented IC 50 of 0.14 ± 0.08 and 6.41 ± 0.64 μg/mL, respectively, against intraerythrocytic forms of Plasmodium falciparum with CC 50 cytotoxicity values against HepG2 cells of 43.64 ± 7.94 and >150 μg/mL, respectively. The biotechnological potential of these substances in relation to leishmaniasis, Chagas disease and malaria should be more deeply investigated. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
01418130
Volume :
102
Database :
Academic Search Index
Journal :
International Journal of Biological Macromolecules
Publication Type :
Academic Journal
Accession number :
124043695
Full Text :
https://doi.org/10.1016/j.ijbiomac.2017.04.013