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Challenges to Design and Develop of DNA Aptamers for Protein Targets. II. Development of the Aptameric Affinity Ligands Specific to Human Plasma Coagulation Factor VIII Using SEC-SELEX.
- Source :
-
Iranian Journal of Pharmaceutical Research . Spring2017, Vol. 16 Issue 2, p734-741. 8p. - Publication Year :
- 2017
-
Abstract
- Protein specific aptamers are highly applicable affinity ligands in different fields of research and clinical applications. They have been developed against various targets, in particular, biomacromolecules such as proteins. Among human proteins, the coagulation factors are the most attractive targets for aptamer selection and their specific aptamers have valuable characteristics in therapeutic and analytical applications. In this study, a plasma derived coagulation factor VIII was considered as the protein target for DNA aptamer selection using size exclusion chromatography-SELEX. Potential aptameric oligonucleotides with high affinity and specificity were achieved during eight rounds of selection. Binding affinity constant of selected aptamer and aptameric enriched pool were in nanomolar range that was comparable to monoclonal antibodies. Further improvement studies can result in aptamers that are more promising as an industrial affinity ligand for the purification of anti-hemophilia factor from plasma source. [ABSTRACT FROM AUTHOR]
- Subjects :
- *BLOOD coagulation factors
*COLLOIDS
*BLOOD coagulation
*DEOXYRIBOSE
*DNA
Subjects
Details
- Language :
- English
- ISSN :
- 17350328
- Volume :
- 16
- Issue :
- 2
- Database :
- Academic Search Index
- Journal :
- Iranian Journal of Pharmaceutical Research
- Publication Type :
- Academic Journal
- Accession number :
- 124017694