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Histone demethylase KDM5A regulates the ZMY ND8-NuRD chromatin remodeler to promote DNA repair.
- Source :
-
Journal of Cell Biology . Jul2017, Vol. 216 Issue 7, p1959-1974. 16p. - Publication Year :
- 2017
-
Abstract
- Upon DNA damage, histone modifications are dynamically reshaped to accommodate DNA damage signaling and repair within chromatin. In this study, we report the identification of the histone demethylase KDM5A as a key regulator of the bromodomain protein ZMY ND8 and NuRD (nucleosome remodeling and histone deacetylation) complex in the DNA damage response. We observe KDM5A-dependent H3K4me3 demethylation within chromatin near DNA double-strand break (DSB) sites. Mechanistically, demethylation of H3K4me3 is required for ZMY ND8-NuRD binding to chromatin and recruitment to DNA damage. Functionally, KDM5A deficiency results in impaired transcriptional silencing and repair of DSBs by homologous recombination. Thus, this study identifies a crucial function for KDM5A in demethylating H3K4 to allow ZMY ND8-NuRD to operate within damaged chromatin to repair DSBs. [ABSTRACT FROM AUTHOR]
- Subjects :
- *HISTONE demethylases
*DNA repair
*CHROMATIN-remodeling complexes
Subjects
Details
- Language :
- English
- ISSN :
- 00219525
- Volume :
- 216
- Issue :
- 7
- Database :
- Academic Search Index
- Journal :
- Journal of Cell Biology
- Publication Type :
- Academic Journal
- Accession number :
- 124003580
- Full Text :
- https://doi.org/10.1083/jcb.201611135