多囊肾病小型猪肾脏组织中增殖和凋亡的改变.

Objective: To observe proliferation and apoptosis of the cell in the kidney of Mini-Pigs with ADPKD.Methods: We observed the protein expression changes of cell proliferation marker PCNA, proliferation-related mTOR signal pathway seed molecules (phospho-mTOR, phospho-p70S6 (Phospho-PKK, phospho-MEK, phospho-ERK), endothelial cell adhesion molecule-1 (CD31), Bax, Bcl-2 and caspase 3 in the kidney tissues of PKD1 Deletion mini-pig model by West-ern blot and immunohistochemical staining.Results: The results showed that the level of PCNA was markedly up-regulated.The molecules in mTOR signal pathway (phospho-mTOR, phospho-p70S6, phospho-4EBP1) ERK signal pathway (phospho-PKA, phospho-MEK, phospho-ERK) and CD3 lwere markedly increased in ADPKD mini-pig kidney tissues. Addition, the Bax / Bcl-2ratio and the caspase3 were increased significantly.Conclusion: The research shown Which the signal pathway of proliferation and apoptosis was activated in ADPKD mini-pig Kidney tissues. [ABSTRACT FROM AUTHOR]