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Oas1b-dependent Immune Transcriptional Profiles of West Nile Virus Infection in the Collaborative Cross.

Authors :
Green, Richard
Wilkins, Courtney
Thomas, Sunil
Sekine, Aimee
Hendrick, Duncan M.
Voss, Kathleen
Ireton, Renee C.
Mooney, Michael
Go, Jennifer T.
Choonoo, Gabrielle
Jeng, Sophia
de Villena, Fernando Pardo-Manuel
Ferris, Martin T.
McWeeney, Shannon
Gale Jr., Michael
Source :
G3: Genes | Genomes | Genetics. Jun2017, Vol. 7 Issue 6, p1665-1682. 18p.
Publication Year :
2017

Abstract

The oligoadenylate-synthetase (Oas) gene locus provides innate immune resistance to virus infection. In mouse models, variation in the Oas1b gene influences host susceptibility to flavivirus infection. However, the impact of Oas variation on overall innate immune programming and global gene expression among tissues and in different genetic backgrounds has not been defined. We examined how Oas1b acts in spleen and brain tissue to limit West Nile virus (WNV) susceptibility and disease across a range of genetic backgrounds. The laboratory founder strains of the mouse Collaborative Cross (CC) (A/J, C57BL/6J, 129S1/SvImJ, NOD/ShiLtJ, and NZO/HlLtJ) all encode a truncated, defective Oas1b, whereas the three wild-derived inbred founder strains (CAST/EiJ, PWK/PhJ, and WSB/EiJ) encode a full-length OAS1B protein. We assessed disease profiles and transcriptional signatures of F1 hybrids derived from these founder strains. F1 hybrids included wild-type Oas1b (F/F), homozygous null Oas1b (N/N) and heterozygous offspring of both parental combinations (F/N and N/F). These mice were challenged with WNV, and brain and spleen samples were harvested for global gene expression analysis. We found that the Oas1b haplotype played a role in WNV susceptibility and disease metrics, but the presence of a functional Oas1b allele in heterozygous offspring did not absolutely predict protection against disease. Our results indicate that Oas1b status as wild-type or truncated, and overall Oas1b gene dosage, link with novel innate immune gene signatures that impact specific biological pathways for the control of flavivirus infection and immunity through both Oas1b-dependent and independent processes. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
21601836
Volume :
7
Issue :
6
Database :
Academic Search Index
Journal :
G3: Genes | Genomes | Genetics
Publication Type :
Academic Journal
Accession number :
123783451
Full Text :
https://doi.org/10.1534/g3.117.041624