携抗 MUC1 单克隆抗体的超声造影剂微泡制备及体外寻靶实验.

Objective: To prepare ultrasound contrast agent microbubbles combining anti-MUCl monoclone antibody and evaluate its targeting capability in vitro. Methods: Ultrasound contrast agent microbubbles and anti-MUCl monoclone antibodies were combined via N-succinimide 3-(2-pyridyl dithio) propionate (SPDP). Laser particle size analyzer, scanning electron microscope (SEM) and laser scanning confocal microscope (LSCM) were used to assess the diameter, morphology and ratio of combination between microbubbles and antibodies. Mice EMT6 breast cancer cells were cultured in vitro, which were then mixed with MUCl-targeted microbubbles. Adhension rate were evaluated via LSCM. Results: SEM showed MUCl-antibody carrying microbubbles were regular spherical particles with the diameter of 2.88 ± 1.34 u.m. Via SPDP, MUC1-antibodies were conjugated onto the surface of microbubbles with the ratio of 77.3 ± 10.4% by LSCM. Adhension rate was 79.2 ± 13.2%, when MUCl-antibody carrying microbubbles were mixed with Mice EMT6 breast cancer cells cultured in vitro. Conclusions: MUCl-antibody carrying microbubbles were prepared successfully, and could adhere to breast cancer cells in vitro. [ABSTRACT FROM AUTHOR]