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Association of peripheral NK cell counts with Helios+IFN-γ- Tregs in patients with good long-term renal allograft function.
- Source :
-
Clinical & Experimental Immunology . Jun2017, Vol. 188 Issue 3, p467-479. 14p. 4 Charts, 3 Graphs. - Publication Year :
- 2017
-
Abstract
- Little is known about a possible interaction of natural killer (NK) cells with regulatory T cells (Treg) in long-term stable kidney transplant recipients. Absolute counts of lymphocyte and Treg subsets were studied in whole blood samples of 136 long-term stable renal transplant recipients and 52 healthy controls using eight-colour fluorescence flow cytometry. Patients were 1946 ± 2201 days (153-10 268 days) post-transplant and showed a serum creatinine of 1·7 ± 0·7 mg/dl. Renal transplant recipients investigated > 1·5 years post-transplant showed higher total NK cell counts than recipients studied < 1·5 years after transplantation ( P = 0·006). High NK cells were associated with high glomerular filtration rate ( P = 0·002) and low serum creatinine ( P = 0·005). Interestingly, high NK cells were associated with high CD4+CD25+CD127-forkhead box protein 3 (FoxP3+) Treg that co-express the phenotype Helios+interferon (IFN)-γ- and appear to have stable FoxP3 expression and originate from the thymus. Furthermore, high total NK cells were associated with Treg that co-express the phenotypes interleukin (IL)−10-transforming growth factor (TGF)-β+ ( P = 0·013), CD183+CD62L- ( P = 0·003), CD183+CD62+( P = 0·001), CD183-CD62L+ ( P = 0·002), CD252-CD152+ ( P < 0·001), CD28+human leucocyte antigen D-related (HLA-DR-) ( P = 0·002), CD28+HLA-DR+ ( P < 0·001), CD95+CD178- ( P < 0·001) and CD279-CD152+ ( P < 0·001), suggesting that these activated Treg home in peripheral tissues and suppress effector cells via TGF-β and cytotoxic T lymphocyte-associated protein 4 (CTLA-4). The higher numbers of NK and Treg cell counts in patients with long-term good allograft function and the statistical association of these two lymphocyte subsets with each other suggest a direct or indirect (via DC) interaction of these cell subpopulations that contributes to good long-term allograft acceptance. Moreover, we speculate that regulatory NK cells are formed late post-transplant that are able to inhibit graft-reactive effector cells. [ABSTRACT FROM AUTHOR]
- Subjects :
- *KIDNEY transplantation
*KILLER cells
*T cells
*FLOW cytometry
*IMMUNOSUPPRESSION
Subjects
Details
- Language :
- English
- ISSN :
- 00099104
- Volume :
- 188
- Issue :
- 3
- Database :
- Academic Search Index
- Journal :
- Clinical & Experimental Immunology
- Publication Type :
- Academic Journal
- Accession number :
- 122941787
- Full Text :
- https://doi.org/10.1111/cei.12945