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Rapid Determination of Isomeric Benzoylpaeoniflorin and Benzoylalbiflorin in Rat Plasma by LC-MS/MS Method.
- Source :
-
International Journal of Analytical Chemistry . 5/8/2017, p1-9. 9p. - Publication Year :
- 2017
-
Abstract
- Benzoylpaeoniflorin (BP) is a potential therapeutic agent against oxidative stress related Alzheimer’s disease. In this study, a more rapid, selective, and sensitive liquid chromatography-tandem mass spectrometric (LC-MS/MS) method was developed to determine BP in rat plasma distinguishing with a monoterpene isomer, benzoylalbiflorin (BA). The method showed a linear response from 1 to 1000 ng/mL (r>0.9950). The precision of the interday and intraday ranged from 2.03 to 12.48% and the accuracy values ranged from −8.00 to 10.33%. Each running of the method could be finished in 4 minutes. The LC-MS/MS method was validated for specificity, linearity, precision, accuracy, recovery, and stability and was found to be acceptable for bioanalytical application. Finally, this fully validated method was successfully applied to a pharmacokinetic study in rats following oral administration. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 16878760
- Database :
- Academic Search Index
- Journal :
- International Journal of Analytical Chemistry
- Publication Type :
- Academic Journal
- Accession number :
- 122918974
- Full Text :
- https://doi.org/10.1155/2017/1693464