急性酒精中毒合并中度创伤性脑损伤对大鼠海马NOS2表达和学习记忆的影响.

Objective: To investigate the spatial memory and the expression of hippocampal NOS2 in rat model of acute alcohol intoxication combined with TBI. Methods: After practice in water maze,96 male SD rats were randomly divided into four groups: N group（saline injection）,A group（alcohol injection）,T group（traumatic brain injury and saline injection） and AT group（traumatic brain injury and alcohol injection）. The animal model of acute alcohol intoxication with TBI was established by hitting rat forehead vertically 2hours following injecting of 25% alcohol 2.5 g/kg intraperitoneally. Animals of each group sacrificed at 1,3,5,7,14 days after behavior tests respectively. The spatial memory was tested by Morris-maze. The expression of NOS2 in hippocampus neurons was detected by immunohistochemistry method. Results: All experimental groups exhibit higher expression of NOS2 than that of N control group. NOS2 expression in T group was higher than in AT group at the 1stday after surgery（P<0.01）. NOS2 expression in AT group was higher than that of T group from 5thday to14thday after surgery（P<0.05）. In water maze tests,latency in T group was shorter than that in AT group（P<0.05） in the1stand the 14thday. However,in the 3rdday,latency in NT group was longer than in AT group（P<0.05）. Conclusions: Acute alcohol intoxication with advanced TBI had long latency in finding the platform,suggesting that capacity of spatial learning and memory was impaired. The increased expression of NOS2 in rat CA1 neurons area of hippocampus may contribute to secondary brain injury,and may be one of the reasons for unfavourable prognosis in later stage. [ABSTRACT FROM AUTHOR]