Determination of Morphine by Capillary Zone Electrophoresis Immunoassay Combined with Laser-Induced Fluorescence Detection.

A competitive immunoassay for detecting morphine in bio-samples was established by capillary zone electrophoresis combined with laser-induced fluorescence detection (CZE-LIF). The antigen of morphine was labeled with isothiocyano-fluorescein (FITC) and then incubated with morphine monoclonal antibody and samples. The linear range was 50–1000 ng/mL, which was suitable for clinical and forensic applications. The detection limit can reach 40 ng/mL, based on S/N = 2. The recoveries of morphine from serum were satisfactory. [ABSTRACT FROM AUTHOR]