Back to Search
Start Over
PUGNAc treatment provokes globotetraosylceramide accumulation in human umbilical vein endothelial cells.
- Source :
-
Biochemical & Biophysical Research Communications . May2017, Vol. 487 Issue 1, p76-82. 7p. - Publication Year :
- 2017
-
Abstract
- PUGNAc is a well-investigated inhibitor for protein- O -GlcNAcase, whereas recent investigations showed that PUGNAc had a broad range as inhibitor for cellular β-hexosaminidases. Here we report that PUGNAc treatment provokes globotetraosylceramide (Gb4Cer) accumulation in human umbilical vein endothelial cells (HUVEC). HPLC analysis and a quantitative ELISA using newly developed anti-Gb4Cer monoclonal antibody revealed that PUGNAc treatment specifically increased the expression of Gb4Cer among glycosphingolipids expressed in HUVEC. Although the effect was weaker than PUGNAc, an O -GlcNAcase selective inhibitor (Thiamet-G) treatment also increased Gb4Cer levels in HUVEC. Furthermore, both of PUGNAc and Thiamet-G treatment up-regulated the expression levels of α-1,4-galactosyltransferase/Gb3Cer synthase gene which encodes a key enzyme in Gb4Cer synthesis. These results indicate that protein- O -GlcNAcylation can regulate the expression levels of cellular Gb4Cer. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 0006291X
- Volume :
- 487
- Issue :
- 1
- Database :
- Academic Search Index
- Journal :
- Biochemical & Biophysical Research Communications
- Publication Type :
- Academic Journal
- Accession number :
- 122623273
- Full Text :
- https://doi.org/10.1016/j.bbrc.2017.04.019