STUDIES ON THE EFFECT OF CERTAIN ENZYMIC POISONS ON THE METABOLISM OF STORAGE ORGANS VI. ABSORPTION AND UTILIZATION OF HEXOSES BY RADISH ROOT SLICES AS INFLUENCED BY IODACETATE.

Glucose and fructose fed to radish slices were taken up at different relative rates with marked accumulation of carbohydrates; a possible working mechanism is presented to explain the differential rates of uptake. Addition of iodoacetate to the sugar media decreased both the uptake of sugars and the accumulation of carbohydrates. Although there was synthesis of proteins in the presence of iodoacetate, the synthesis both in iodoacetate and in iodoacetate plus sugar was less than in the absence of iodoacetate. Sugars and iodoacetate either alone or in combination increased the rates of CO[SUB2] output; a possible explanation of the different rates observed is given. Moreover, iodoacetate increased the permeability of the cell membranes as is evident from the increased leakage of nitrogen compounds from the tissues into the media. [ABSTRACT FROM AUTHOR]