不同初加工滇龙胆HPLC指纹图谱 及其有效成分含量测定.

HPLC fingerprints of different preliminary processing Gentiana rigescens were established, and 4 index contents as loganic acid, swertimarin, gentiopicroside and swerosidefour were determined. Principal component analysis (PCA) was employed to evaluate nine preliminary processing methods. The results showed relatively high similarity among samples. The contents of swertimarin, gentiopicroside and sweroside were the highest in Gentiana rigescens samples which were cleaned by rinsing and indoor seasoning, and reached(5.89 ±0.23), (15.62±0.84),(3.79 ±0.16)mg/g, respectively. While the sample which was treated by microwave had the highest content of loganic acid, was( 8. 58 ± 1 .48) mg/g. The total content of the 4 effective components was(27. 67 ± 6. 09) mg/g. The results of principal component analysis revealed that cumulativerate of contribution of PC A1 and PC A2 reached 86. 822 %, and formed linearrelation with four effective constituents: F(PC1) =-0.003X1 + 0.409X2 + 0.31X3 + 0.398X4, F(PC2) = 0.875X1 - 0.168X2 + 0. 329X3 - 0.078X4; The results showed that the samples with the skin dried indoor after washing got the highest score in PCA. Analysis results revealed cleaning by rinsing and indoor seasoning was more optimizing processing method than others. [ABSTRACT FROM AUTHOR]