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Evaluation of Breeding Programs Susceptibility for Two Important Forage Crops Using DNA Barcoding.
- Source :
-
Alexandria Science Exchange Journal . Jan-Mar2017, Vol. 38 Issue 1, p27-37. 11p. - Publication Year :
- 2017
-
Abstract
- This investigation was carried out to identify and evaluate Medicago sativa and Trifolium alexandrinum probability for breeding program based on two bar-coding genes (rbcl and Cox1 genes). Identification of Medicago sativa Baladi 1 was performed through rbcl and Cox1 genes. Medicago sativa Baladi 1 was identified as Medicago sativa voucher G00199095 ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit (rbcL) gene, partial cds; chloroplast (Sequence ID: KJ204375.1) and Medicago sativa voucher Ahrendsen_23 for rbcl and Cox1 genes respectively. Identity values were recorded with 90% of identity for alfalfa, Baladi 1 Genotype ribulose - 1/5 - bisphosphate carboxylase / oxygenase large subunit (rbcl) gene (sequences ID: KJ206375.1) also, identity values were recorded with 91.24% of identity for alfalfa Baladi 1 Genotype, cytochrome c oxiddase bubunit I gene (cox 1) (sequence ID: KJ 204375.1). Trifolium alexandrinum Helaly genotype was identified as Trifolium alexandrinum (Sequence ID: HM850407.1) and Trifolium alexandrinum voucher K-016Hv (Sequence ID: KU234213.1) as rbcl and Cox1 genes respectively. Affiliation of genetic origin was detected for Trifolium alexandrinum with 100 % of similarity with origin source which indicate highly possibility for applying breeding programs comparing with Medicago sativa which reflect the lowest genetic similarity with origin source. [ABSTRACT FROM AUTHOR]
- Subjects :
- *GENETIC barcoding
*FORAGE plants
*BERSEEM
Subjects
Details
- Language :
- English
- ISSN :
- 11100176
- Volume :
- 38
- Issue :
- 1
- Database :
- Academic Search Index
- Journal :
- Alexandria Science Exchange Journal
- Publication Type :
- Academic Journal
- Accession number :
- 122194224
- Full Text :
- https://doi.org/10.21608/asejaiqjsae.2017.1619