Back to Search
Start Over
The Vital Dye CDr10b Labels the Zebrafish Mid-Intestine and Lumen.
- Source :
-
Molecules . Mar2017, Vol. 22 Issue 3, p454. 10p. 5 Diagrams. - Publication Year :
- 2017
-
Abstract
- We describe the use of the fluorescent reporter compound CDr10b to label mid-intestinal structures in zebrafish larvae after simple immersion. CDr10b is deposited into the gut where it initially fills the lumen and is excreted. Using laser-mediated injury of the intestine, we show that CDr10b provides a useful readout of the integrity and repair of the epithelial cell barrier. In addition, CDr10b specifically labels the absorptive mid-intestine segment that is analogous to the mammalian small intestine. By perturbing retinoic acid signaling, which regulates the size of the mid-intestine segment, we show that CDr10b is a valuable tool to rapidly assess developmental malformations of the intestine in live animals. [ABSTRACT FROM AUTHOR]
- Subjects :
- *DYES & dyeing
*ZEBRA danio
*SMALL intestine
*EPITHELIAL cells
FISH digestive organs
Subjects
Details
- Language :
- English
- ISSN :
- 14203049
- Volume :
- 22
- Issue :
- 3
- Database :
- Academic Search Index
- Journal :
- Molecules
- Publication Type :
- Academic Journal
- Accession number :
- 122142152
- Full Text :
- https://doi.org/10.3390/molecules22030454