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Ectopic expression of TaOEP16-2-5B, a wheat plastid outer envelope protein gene, enhances heat and drought stress tolerance in transgenic Arabidopsis plants.
- Source :
-
Plant Science . May2017, Vol. 258, p1-11. 11p. - Publication Year :
- 2017
-
Abstract
- Abiotic stresses, such as heat and drought, are major environmental factors restricting crop productivity and quality worldwide. A plastid outer envelope protein gene, TaOEP16-2 , was identified from our previous transcriptome analysis [1,2] . In this study, the isolation and functional characterization of the TaOEP16-2 gene was reported. Three homoeologous sequences of TaOEP16-2 were isolated from hexaploid wheat, which were localized on the chromosomes 5A, 5B and 5D, respectively. These three homoeologues exhibited different expression patterns under heat stress conditions, TaOEP16-2 - 5B was the dominant one, and TaOEP16-2 - 5B was selected for further analysis. Compared with wild type (WT) plants, transgenic Arabidopsis plants overexpressing the TaOEP16-2 - 5B gene exhibited enhanced tolerance to heat stress, which was supported by improved survival rate, strengthened cell membrane stability and increased sucrose content. It was also found that TaOEP16-2 was induced by drought stress and involved in drought stress tolerance. TaOEP16-2-5B has the same function in ABA-controlled seed germination as AtOEP16-2 . Our results suggest that TaOEP16-2-5B plays an important role in heat and drought stress tolerance, and could be utilized in transgenic breeding of wheat and other crop plants. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 01689452
- Volume :
- 258
- Database :
- Academic Search Index
- Journal :
- Plant Science
- Publication Type :
- Academic Journal
- Accession number :
- 121937000
- Full Text :
- https://doi.org/10.1016/j.plantsci.2017.01.011