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雌激素对肺腺癌细胞系A549 中NOK表达量的影响.
- Source :
-
Progress in Modern Biomedicine . 2016, Vol. 16 Issue 34, p6618-6621. 4p. - Publication Year :
- 2016
-
Abstract
- Objective: To study the effect of estrogen on the expression of NOK in lung adenocarcinoma cell line A549, and to provide a basis for studying the role of estrogen and NOK gene in non small cell lung cancer metastasis. Methods: MTT method was used to draw the growth curve of lung adenocarcinoma cell line A549, then determine the exponential growth phase of A549. To stimulate cells with estrogen which concentration gradient is 1× 10-10-1× 10-6mol/L in exponential growth phase. The cells were cultured in routine medium 12 h later. Then, RNA and protein were extracted. The expression of NOK was tested by q-RT-PCR and Western blot. Results: The exponential growth phase of A549 is the third day; NOK expression generally increased after estrogen stimulating, the expression of NOK increased with the increase of estrogen concentration in the low concentration, the expression of NOK decreased with the increase of estrogen concentration in the high concentration, but still higher than normal level. The NOK expression of the estrogen groups were significantly higher than that of the control group. Among them, the NOK expression was the highest when the estrogen concentration was 1× 10-8mol/L. Conclusions: In a certain concentration range, estrogen can promote the expression of NOK in lung adenocarcinoma cell line A549. [ABSTRACT FROM AUTHOR]
Details
- Language :
- Chinese
- ISSN :
- 16736273
- Volume :
- 16
- Issue :
- 34
- Database :
- Academic Search Index
- Journal :
- Progress in Modern Biomedicine
- Publication Type :
- Academic Journal
- Accession number :
- 121906508
- Full Text :
- https://doi.org/10.13241/j.cnki.pmb.2016.34.005