Assignment<FOOTREF>[sup 1] </FOOTREF> of the cellular retinol-binding protein 1 gene (RBP1) and of the coatomer beta> subunit gene (COPB2) to human chromosome band 3q23 by in situ hybridization.

This article describes the mapping of the cellular retinol-binding protein 1 gene (RBP1) and of the coatomer beta subunit gene (COPB2) to human chromosome band 3q23 by in situ hybridization. The human cellular retinol-binding protein 1 (RBP 1) is responsible for the intracellular transport of vitamin A to the final site of action. The DNA of the PAC clones was prepared using an alkaline lysis mini- prep method and labeled with digoxigenin-1 1-dUTP (0.4 mM, Boehringer) by nick translation. The PACs were cohybridized with a biotinylated probe for the centromeric region of chromosome 3.