19 - New Insights on Effects of a Dietary Supplement Utilizing the "Redox Stress Hypothesis".

The research community is generally agreed that maintenance of healthy levels of free radicals and related oxidants are important for good health. However, utilization of the “redox stress hypothesis” can provide us with concrete nutritional targets in order to better support and maintain “optimal health.” Following this hypothesis, we performed a single-dose study on the effects of dietary supplement Spectra5 TM on oxidative and nitrosative stress markers as well as metabolic, inflammatory and cardiovascular parameters in human participants (n=8). The measurements of ex vivo intra- and extracellular formation of reactive oxygen species (ROS, O 2 – , H 2 O 2 , OH) in whole blood, respiratory activity of blood cells, as well as mitochondrial-dependent ROS formation, and respiratory activity were performed using EPR spectrometer NOXYSCAN, spin probe CMH, and oxygen label NOX-15.1, respectively. Furthermore, we investigated the ability of supplement to modulate ex vivo cellular inflammatory responses induced by stimulation with exogenous TNF-α and also followed changes in bioavailable NO concentrations. In this study, we demonstrated that administration of single dose Spectra5 TM resulted in statistically significant long-term, time depended inhibition of mitochondrial and cellular ROS generation by as much as 13% as well as 2.5-times inhibition in extracellular NADPH system-dependent generation of O 2 – , and nearly complete inhibition of extracellular H 2 O 2 formation. This was reflected in more significant inhibition of ex-vivo cellular inflammatory response and also increases in bioavailable NO concentration. In this study we have measured synergetic, biological effects of a natural supplement on changes in oxidative and nitrosative stress as well as cellular metabolic activity. The unique design and activity of the plant-based natural supplement, in combination with the validated “Extended Vitality Test”, demonstrates the efficiency to use the dietary supplements by utilizing the “redox stress hypothesis”. [ABSTRACT FROM AUTHOR]