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Factors affecting induction of peripheral IFN-γ recall response to influenza A virus vaccination in pigs.
- Source :
-
Veterinary Immunology & Immunopathology . Mar2017, Vol. 185, p57-65. 9p. - Publication Year :
- 2017
-
Abstract
- While T cell contribution to IAV immunity is appreciated, data comparing methods to evaluate IFN-γ production by IAV-specific T cells elicited following vaccination is limited. To understand the differential immunogenicity between live-attenuated influenza virus (LAIV) and whole-inactivated virus (WIV) vaccines in relation to induction of peripheral T cell responses, ELISpot and ELISA were used to assess IFN-γ production by peripheral lymphocytes following antigen restimulation. Following restimulation, peripheral blood lymphocytes from WIV-vaccinated pigs had a greater quantity of IFN-γ secreting cells (SC) and IFN-γ secreted compared to LAIV vaccinated and non-vaccinated (NV) pigs. Pig age at time of WIV vaccination significantly impacted peripheral IAV-specific IFN-γ recall response, as did the inclusion of adjuvant in the WIV vaccine. Collectively, these data indicate that peripheral IAV-specific IFN-γ recall responses are not predictive of LAIV vaccination status, thus, are unlikely to be a useful surrogate for evaluating LAIV immunogenicity and predicting cross-protection. However, these data suggest that the evaluation of peripheral IFN-γ recall responses may be useful for identifying factors, such as animal age or vaccine formulation, that may impact parenterally-delivered WIV vaccine immunogenicity. Overall, results did not differ based upon the assay used to evaluate IFN-γ recall responses. Therefore, either ELISpot or ELISA could serve as a measure for evaluating IAV-specific IFN-γ cell-mediated immune responses in swine. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 01652427
- Volume :
- 185
- Database :
- Academic Search Index
- Journal :
- Veterinary Immunology & Immunopathology
- Publication Type :
- Academic Journal
- Accession number :
- 121452723
- Full Text :
- https://doi.org/10.1016/j.vetimm.2017.01.009