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Roles of microRNA-330 and Its Target Gene ING4 in the Development of Aggressive Phenotype in Hepatocellular Carcinoma Cells.

Authors :
Hu, Xiao
Feng, Yujie
Sun, Lin
Qu, Linlin
Sun, Chuandong
Source :
Digestive Diseases & Sciences. Mar2017, Vol. 62 Issue 3, p715-722. 8p.
Publication Year :
2017

Abstract

<bold>Background: </bold>Aberrant expression of microRNAs contributes to tumor growth and progression.<bold>Aims: </bold>This study was designed to explore the prognostic and biological significance of miR-330 in hepatocellular carcinoma (HCC).<bold>Methods: </bold>The expression of miR-330 and its associations with tumor parameters and overall survival were analyzed in HCC patients. The biological functions of miR-330 in HCC cell growth, invasion, and tumorigenesis were investigated. Bioinformatic analysis and luciferase reporter assays were performed to search for potential targets of miR-330.<bold>Results: </bold>The miR-330 level was significantly higher in HCCs than in adjacent normal tissues (P = 0.0085). High expression of miR-330 was significantly associated with more aggressive phenotypes and shorter overall survival in HCC. Loss- and gain-of-function studies indicated the favorable effect of miR-330 on tumor cell growth, invasion, and tumorigenesis. Inhibitor of growth 4 (ING4) was identified to be a direct target of miR-330. Overexpression of miR-330 reduced the expression of ING4 in HCC cells. Importantly, restoration of ING4 almost completely reversed the promotion of HCC cell proliferation and invasion by miR-330.<bold>Conclusions: </bold>Altogether, this study demonstrates that upregulation of miR-330 is associated with poor prognosis and contributes to more aggressive phenotypes of HCC. The oncogenic role of miR-330 in HCC is linked to downregulation of ING4. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
01632116
Volume :
62
Issue :
3
Database :
Academic Search Index
Journal :
Digestive Diseases & Sciences
Publication Type :
Academic Journal
Accession number :
121387077
Full Text :
https://doi.org/10.1007/s10620-016-4429-2