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Roles of microRNA-330 and Its Target Gene ING4 in the Development of Aggressive Phenotype in Hepatocellular Carcinoma Cells.
- Source :
-
Digestive Diseases & Sciences . Mar2017, Vol. 62 Issue 3, p715-722. 8p. - Publication Year :
- 2017
-
Abstract
- <bold>Background: </bold>Aberrant expression of microRNAs contributes to tumor growth and progression.<bold>Aims: </bold>This study was designed to explore the prognostic and biological significance of miR-330 in hepatocellular carcinoma (HCC).<bold>Methods: </bold>The expression of miR-330 and its associations with tumor parameters and overall survival were analyzed in HCC patients. The biological functions of miR-330 in HCC cell growth, invasion, and tumorigenesis were investigated. Bioinformatic analysis and luciferase reporter assays were performed to search for potential targets of miR-330.<bold>Results: </bold>The miR-330 level was significantly higher in HCCs than in adjacent normal tissues (P = 0.0085). High expression of miR-330 was significantly associated with more aggressive phenotypes and shorter overall survival in HCC. Loss- and gain-of-function studies indicated the favorable effect of miR-330 on tumor cell growth, invasion, and tumorigenesis. Inhibitor of growth 4 (ING4) was identified to be a direct target of miR-330. Overexpression of miR-330 reduced the expression of ING4 in HCC cells. Importantly, restoration of ING4 almost completely reversed the promotion of HCC cell proliferation and invasion by miR-330.<bold>Conclusions: </bold>Altogether, this study demonstrates that upregulation of miR-330 is associated with poor prognosis and contributes to more aggressive phenotypes of HCC. The oncogenic role of miR-330 in HCC is linked to downregulation of ING4. [ABSTRACT FROM AUTHOR]
- Subjects :
- *MICRORNA
*LIVER cancer
*TUMOR growth
*CANCER invasiveness
*GENE expression
*CELL proliferation
*BIOCHEMISTRY
*CELL lines
*CELL physiology
*GENES
*HEPATOCELLULAR carcinoma
*LIVER tumors
*PHENOMENOLOGY
*PROGNOSIS
*PROTEINS
*RNA
*SURVIVAL analysis (Biometry)
*TUMOR classification
*DISEASE progression
*CELL cycle proteins
*DIAGNOSIS
Subjects
Details
- Language :
- English
- ISSN :
- 01632116
- Volume :
- 62
- Issue :
- 3
- Database :
- Academic Search Index
- Journal :
- Digestive Diseases & Sciences
- Publication Type :
- Academic Journal
- Accession number :
- 121387077
- Full Text :
- https://doi.org/10.1007/s10620-016-4429-2