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Dexmedetomidine Attenuates Lipopolysaccharide Induced MCP-1 Expression in Primary Astrocyte.
- Source :
-
BioMed Research International . 2/14/2017, Vol. 2017, p1-6. 6p. - Publication Year :
- 2017
-
Abstract
- Background. Neuroinflammation which presents as a possible mechanism of delirium is associated with MCP-1, an important proinflammatory factor which is expressed on astrocytes. It is known that dexmedetomidine (DEX) possesses potent anti-inflammatory properties. This study aimed to investigate the potential effects of DEX on the production of MCP-1 in lipopolysaccharide-stimulated astrocytes. Materials and Methods. Astrocytes were treated with LPS (10 ng/ml, 50 ng/ml, 100 ng/ml, and 1000 ng/ml), DEX (500 ng/mL), LPS (100 ng/ml), and DEX (10, 100, and 500 ng/mL) for a duration of three hours; expression levels of MCP-1 were measured by real-time PCR. The double immunofluorescence staining protocol was utilized to determine the expression of α2-adrenoceptors (α2AR) and glial fibrillary acidic protein (GFAP) on astrocytes. Results. Expressions of MCP-1 mRNA in astrocytes were induced dose-dependently by LPS. Administration of DEX significantly inhibited the expression of MCP-1 mRNA (P<0.001). Double immunofluorescence assay showed that α2AR colocalize with GFAP, which indicates the expression of α2-adrenoceptors in astrocytes. Conclusions. DEX is a potent suppressor of MCP-1 in astrocytes induced with lipopolysaccharide through α2A-adrenergic receptors, which potentially explains its beneficial effects in the treatment of delirium by attenuating neuroinflammation. [ABSTRACT FROM AUTHOR]
- Subjects :
- *ANTI-inflammatory agents
*ADRENERGIC receptors
*ANIMAL experimentation
*BIOLOGICAL assay
*CELL culture
*CHEMOKINES
*CYTOSKELETAL proteins
*DELIRIUM
*DOSE-effect relationship in pharmacology
*ELECTROPHORESIS
*FLUORESCENT antibody technique
*IMIDAZOLES
*INFLAMMATION
*NEUROLOGICAL disorders
*NEUROGLIA
*POLYMERASE chain reaction
*PROBABILITY theory
*RATS
*T-test (Statistics)
*DATA analysis software
*LIPOPOLYSACCHARIDES
*DESCRIPTIVE statistics
*ONE-way analysis of variance
*THERAPEUTICS
Subjects
Details
- Language :
- English
- ISSN :
- 23146133
- Volume :
- 2017
- Database :
- Academic Search Index
- Journal :
- BioMed Research International
- Publication Type :
- Academic Journal
- Accession number :
- 121269744
- Full Text :
- https://doi.org/10.1155/2017/6352159