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EVALUACIÓN DE BACTERIAS ÁCIDO LÁCTICAS Y BACTERIAS ESPORULADAS CON ACTIVIDAD PROBIÓTICA DEL TRACTO GASTROINTESTINAL DE TILAPIA ROJA (Oreochromis sp).

EVALUACIÓN DE BACTERIAS ÁCIDO LÁCTICAS Y BACTERIAS ESPORULADAS CON ACTIVIDAD PROBIÓTICA DEL TRACTO GASTROINTESTINAL DE TILAPIA ROJA (Oreochromis sp).

Authors :
GUTIERREZ RAMIREZ, Luz Adriana
DAVID RUALES, Carlos Arturo
NARANJO, Ricardo GARCIA
Source :
Vitae (01214004). 2016 Supplement, Vol. 23, pS34-S35. 2p.
Publication Year :
2016

Abstract

Background: Probiotics are living microorganisms, favoring the consumer's health when consumed in adequate amounts. Probiotic characterization evaluated the capacity that some microorganisms such as Bacillus sp and lactic acid bacteria survive different stress conditions, simulating the intestinal conditions: bile salts of 0.3%, pH 2.5, enzymes proteolytic and pH alkaline among others. Objectives: To isolate, characterize and evaluate strains of lactic acid bacteria and bacteria spore with potential probiotic from the gastrointestinal tract of red tilapia (Oreochromis sp). Methods: 20 samples of gastrointestinal tissue were processed in selective culture media for lactic acid bacterias (M17 Agar, MRS Agar) and sporulated bacterias (nutrient Agar). To evaluate the probiotic capacity, assays of tolerance to acid and alkaline pH (2.5 and 8.0), resistance to the action bile salts (0.3%), resistance to a hypertonic medium (NaCl 2.5%), survival to enzymes (lysozyme), hemolytic activity and sensitivity to antibiotics, were used, followed by biochemical test and molecular identification. Resultads: Six isolates with the best probiotic activity were obtained, three identified as Lactococcus lactis, Lactobacillus delbuckii sub bulgaricus and Lactococcus lactis and three isolates of Bacillus sp identified as Bacillus megaterium, Bacillus sphaericus and Bacillus polimyxa. Conclusions: Results showed that in the Tilapia intestine coexists microorganism comunities, especially Bacillus sp and portions of smaller lactic acid bacterias susceptible to be characterized as probiotic. [ABSTRACT FROM AUTHOR]

Details

Language :
Spanish
ISSN :
01214004
Volume :
23
Database :
Academic Search Index
Journal :
Vitae (01214004)
Publication Type :
Academic Journal
Accession number :
121239177