重组人白细胞介素-11 对肺腺癌 A549 细胞增殖、迁移和侵袭的影响.

Objective To observe the effects of recombinant human interleukin 11(rhIL-11) on proliferation, migration and invasion of A549 cells, and the mechanism thereof. Methods Final concentrations of 0, 10, 20, 50 and 100 μg/L rhIL- 11 were added into pulmonary adenocarcinoma A549 cells. The cell proliferation was detected by MTT. The wound-healing, transwell migration assay were used to validate the capability of the migration and invasion of A549 cells. Matrix metalloproteinases (MMP)-2 and MMP-9 protein expressions were revealed by Western blot assay. Results The proliferation of A549 cells was not significantly changed by rhIL-11. The cell capability to migrate and invade was significantly increased 24 h after treatment with rhIL-11 (P < 0.05). The expression levels of MMP-2 and MMP-9 were significantly un-regulated, and which were increased with the increased concentrations of rhIL-11 (P < 0.05). Conclusion rhIL-11 can promote the migration and invasion of A549 cells, and the up-regulation of MMP-2 and MMP-9 expression might be one of the mechanisms. [ABSTRACT FROM AUTHOR]