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Monitoring of vancomycin in human plasma via portable microchip electrophoresis with contactless conductivity detector and multi-stacking strategy.
- Source :
-
Journal of Chromatography A . Feb2017, Vol. 1485, p142-146. 5p. - Publication Year :
- 2017
-
Abstract
- A portable microchip electrophoresis (MCE) coupled with on-chip contactless conductivity detection (C 4 D) system was evaluated for the determination of vancomycin in human plasma. In order to enhance the detection sensitivity, a new online multi-stacking preconcentration technique based on field-enhanced sample injection (FESI) and micelle-to-solvent stacking (MSS) was developed and implemented in MCE-C 4 D system equipped with a commercially available double T-junction glass chip. The cationic analytes from the two sample reservoirs were injected under FESI conditions and subsequently focused by MSS within the sample-loading channel. The proposed multi-stacking strategy was verified under a fluorescence microscope using Rhodamine 6G as the model analyte and a sensitivity enhancement factor (SEF) of up to 217 was achieved. The developed approach was subsequently implemented in the aqueous-based MCE, coupled to C 4 D in order to monitor the targeted antibiotic (in this case, vancomycin) present in human plasma samples. The multi-stacking and analysis time for vancomycin were 50 s and 250 s respectively, with SEF of approximately 83 when compared to typical gated injection. The detection limit of the method for vancomycin was 1.2 μg/mL, with intraday and interday repeatability RSDs of 2.6% and 4.3%, respectively. Recoveries in spiked human plasma were 99.0%–99.2%. [ABSTRACT FROM AUTHOR]
- Subjects :
- *VANCOMYCIN
*MICROCHIP electrophoresis
*PLASMA gases
*ANTIBIOTICS
*ELECTROPHORESIS
Subjects
Details
- Language :
- English
- ISSN :
- 00219673
- Volume :
- 1485
- Database :
- Academic Search Index
- Journal :
- Journal of Chromatography A
- Publication Type :
- Academic Journal
- Accession number :
- 120954759
- Full Text :
- https://doi.org/10.1016/j.chroma.2017.01.012