Rapid measurement of urease activity using a potentiometric RuO2 pH sensor for detection of Helicobacter pylori.

The feasibility of using a thin-film RuO 2 (ruthenium oxide) pH sensor for the detection of Helicobacter pylori is investigated. In particular, we demonstrate the ability of the sensor to measure urease activity between 1.0 and 50 U/mL in less than 30 s. The developed sensor exhibited a super-Nernstian response of 77.74 mV/pH and excellent reversibility. The pH sensor⿿s ability to detect H. pylori in real time is demonstrated, and found to be much faster than the rapid urease test. [ABSTRACT FROM AUTHOR]