Recombinant expression, characterization, and application of a phospholipase B from Fusarium oxysporum.

In this study, a gene encoding a putative lipase from Fusarium oxysporum was optimized via codon optimization and expressed in Pichia pastoris KM71. The gene product was identified as a phospholipase B (PLB). The engineered P. pastoris was further cultured in a 3.6-L bioreactor. After optimization of the induction conditions, this system produced 6.6 mg mL −1 protein and 6503.8 U mL −1 PLB activity in the culture medium. Efficient expression of this PLB in P. pastoris should reduce the costs of production and application. The purified enzyme, with a specific activity of 1170 U mg −1 , was optimally active at pH 5.0 and 55 °C. The results of a degumming experiment performed using the recombinant PLB showed that the phosphorus content of a test oil was decreased from 75.88 ppm to 3.3 ppm in 2 h under optimal reaction conditions. This study provides a basis for the industrial use of F. oxysporum PLB in oil degumming applications. [ABSTRACT FROM AUTHOR]