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The fast track to canonical Wnt signaling in MC3T3-E1 cells protected by substance P against serum deprivation-induced apoptosis.
- Source :
-
Cell Biology International . Jan2017, Vol. 41 Issue 1, p71-78. 8p. - Publication Year :
- 2017
-
Abstract
- The canonical Wnt pathway is vital to bone physiology by increasing bone mass through elevated osteoblast survival. Although investigated extensively in stem cells, its role in osteoblastic MC3T3-E1 cells has not been completely determined. To explore how this pathway is regulated by different conditions, we assessed the anti-apoptotic effects of substance P on the canonical Wnt pathway in MC3T3-E1 cells by treating cells with serum deprivation or serum starving with 'substance P,' a neuropeptide demonstrated to promote bone growth and stimulate Wnt signaling. The results showed that serum deprivation both induced apoptosis and activated Wnt signal transduction while substance P further stimulated the Wnt pathway via the NK-1 receptor but protected the cells from apoptotic death. Fast-tracking of Wnt signaling by substance P was also noted. These results indicate that nutritional deprivation and substance P synergistically activated the canonical Wnt pathway, a finding that helps to reveal the role of Wnt signaling in bone physiology affected by nutritional deprivation and neuropeptide substance P. [ABSTRACT FROM AUTHOR]
- Subjects :
- *WNT signal transduction
*APOPTOSIS
*SUBSTANCE P
*STEM cells
*SERUM
*NEUROPEPTIDES
Subjects
Details
- Language :
- English
- ISSN :
- 10656995
- Volume :
- 41
- Issue :
- 1
- Database :
- Academic Search Index
- Journal :
- Cell Biology International
- Publication Type :
- Academic Journal
- Accession number :
- 120155782
- Full Text :
- https://doi.org/10.1002/cbin.10676