COPD 大鼠肺组织中小窝蛋白-1 的表达及其与气道重塑的关系.

Objective: To investigate the expression of caveolin-1(Caveolin-1) in the lung tissue of rats with COPD and its correlation with airway remodeling. Methods: 20 cases of 10 week-old Wister rats were randomly divided into the control group and the experimental group, 10 rats in each group. The rats in control group were fed by normal feeding, while the rats in experimental group were fed by the method of smoking and lipopolysaccharide (LPS). All the rats were killed at feeding for 91 days and the lung function were measured. Then immunohistochemical method was used to detect the expression of caveolin-1 in lung tissue, and enzyme-linked immunosorbent adsorption test (ELISA) was used to detect the interleukin IL-8 (IL-8), tumor necrosis factor alpha (TNF-α) and transformation growth factor beta1 (TGF-β1) in lung tissue. The correlation of caveolin-1 expression with IL-8, TNF-αand TGF-β1 expression were analyzed. Results: Compared with the control group, the lung adaptation of rats in experimental group decreased, lung elastic resistance and airway resistance increased, the content of IL-8, TNF-αand TGF-β1 in lung tissue were significantly increased, the expression of caveolin-1 in the lung tissue was significantly decreased (P<0.05). There was negative correlation of the caveolin-1 expression with IL-8, TNF-α, TGF-β1 contents in the lung tissue. Conclusions: The expression of Caveolin-1 in the lung tissue of rats with COPD was significantly decreased, Caveolin-1 might promote the release of inflammatory cytokines and airway remodeling, further participated in the development of COPD. [ABSTRACT FROM AUTHOR]