Site-Directed Mutagenesis of the Saccharomyces cerevisiae Dihydropteroate Synthase FOL1 Gene to Study Pneumocystis jirovecii Mutations in the Orthologue Gene FAS.

Examines the potential resistance of Pneumocystis (P.) jirovecii prophylaxis to sulfa drugs conferred by the double mutation M3, an allele of Saccharomyces cerevisiae dihydropteroate synthase. Failure of P. jirovecii prophylaxis with sulfa drugs; Presence of the double mutation; Integration of the plasmid.