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Isolation and biochemical characterization of a gamma-type phospholipase A2 inhibitor from Macropisthodon rudis snake serum.
- Source :
-
Toxicon . Nov2016, Vol. 122, p1-6. 6p. - Publication Year :
- 2016
-
Abstract
- A novel phospholipaseA 2 (PLA 2 ) inhibitory protein (PLI) was purified from the serum of Macropisthodon rudis , a non-venomous snake mainly found in southern China. The molecular mass of the purified PLI was 160 kDa as determined by Superdex 200HR; however, the PLI protein had only one subunit of 25.4 kDa as determined by 12% SDS-PAGE, indicating an oligomeric protein. PLI cDNA obtained by PCR from the liver of Macropisthodon rudis, revealed 549 bps coding for a mature protein of 183 amino acid residues. Based on an amino acid sequence alignment with venomous and non-venomous snakes, this inhibitor was determined to be in the γ type family of PLI. In vitro experiments showed that PLIγ inhibited enzymatic, inflammatory, and antibacterial activities of snake venom PLA 2 isolated from Agkistrodon acutus. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 00410101
- Volume :
- 122
- Database :
- Academic Search Index
- Journal :
- Toxicon
- Publication Type :
- Academic Journal
- Accession number :
- 119075931
- Full Text :
- https://doi.org/10.1016/j.toxicon.2016.09.011