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Microvirga pakistanensis sp. nov., a novel bacterium isolated from desert soil of Cholistan, Pakistan.
- Source :
-
Archives of Microbiology . Dec2016, Vol. 198 Issue 10, p933-939. 7p. - Publication Year :
- 2016
-
Abstract
- A Gram-negative, non-spore-forming, non-pigmented, strictly aerobic and non-motile short rod bacterium, designated NCCP-1258, was isolated from Cholistan desert soil, Bahawalpur, Pakistan. Growth of strain NCCP-1258 was observed at pH range 6.5-9.5 (optimum 7.5-8.5) and temperature range 20-45 °C (optimum 40 °C), and it tolerated 0-2 % NaCl (optimum 0.5 %, w/v). Phylogenetic analysis based on 16S rRNA gene sequence comparison revealed that strain NCCP-1258 belongs to genus Microvirga and is most closely related to Microvirga lotononidis (98.0 %), Microvirga vignae (97.4 %), Microvirga lupini (97.2 %), Microvirga zambiensis (97.2 %) and Microvirga flocculans (97.1 %). Analysis of the concatenated sequences of four housekeeping gene loci ( dnaK, gyrB, recA and rpoB) also confirmed the placement of strain NCCP-1258 within the genus Microvirga. DNA-DNA relatedness values of NCCP-1258 with above-mentioned type strains were less than 42 %. The DNA G+C content of strain NCCP-1258 was 64.3 mol%. Chemotaxonomic data (predominant menaquinone system was Q-10; major fatty acids were C, C ω7 c and C cyclo ω8 c; the polar lipid profile contained diphosphatidylglycerol, phosphatidylcholine, phosphatidyl dimethyl ethanolamine and phosphatidyl ethanolamine) also supported the affiliation of strain NCCP-1258 to the genus Microvirga. On the basis of physiological and biochemical characteristics, phylogenetic analyses and DNA-DNA relatedness, strain NCCP-1258 can be distinguished from the closely related taxa and thus represents a novel species of the genus Microvirga, for which the name Microvirga pakistanensis sp. nov. is proposed with the type strain NCCP-1258 (=CGMCC 1.15074 = KCTC 42496). [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 03028933
- Volume :
- 198
- Issue :
- 10
- Database :
- Academic Search Index
- Journal :
- Archives of Microbiology
- Publication Type :
- Academic Journal
- Accession number :
- 119060009
- Full Text :
- https://doi.org/10.1007/s00203-016-1251-3