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Determination of Arbutin in Rat Plasma Using Liquid Chromatography-Tandem Mass Spectrometry: Application to a Pharmacokinetic Study After Oral Administration of the Extract of Vaccinium vitis-idaea.
- Source :
-
Journal of Chromatographic Science . Oct2016, Vol. 54 Issue 9, p1508-1513. 6p. - Publication Year :
- 2016
-
Abstract
- A rapid and sensitive bioassay based on liquid chromatography-tandem mass spectrometry (LC-MS-MS) has been developed and validated to measure arbutin in rat plasma. Sample preparation of plasma after the addition of indapamide as internal standard (IS) involved solid-phase extraction (SPE) on C18 cartridges. Reversed-phase chromatography using acetonitrile and 0.5% formic acid solution (pH 2.56) was used for separation in a run time of 4.0 min. The analytes were detected in the negative ion mode using selective reaction monitoring (SRM) of the transitions at m/z 271.2→107.8 for arbutin and 364.3→189.0 for indapamide. The method has the following performance characteristics: a reliable response range of 7.5-5250.0 ng/mL with correlation coefficients (r) of >0.995. The lower limit of quantitation (LLOQ) was 7.5 ng/mL. The intra- and inter-day precision and accuracy of the quality control (QC) samples at low, medium and high concentration levels showed ≤8.79% relative standard deviation (RSD) and −1.15 to 1.49% relative error (RE). The method was successfully applied to a pharmacokinetic study to measure arbutin in rats after extracts of Vaccinium vitis-idaea was orally administered. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 00219665
- Volume :
- 54
- Issue :
- 9
- Database :
- Academic Search Index
- Journal :
- Journal of Chromatographic Science
- Publication Type :
- Academic Journal
- Accession number :
- 118934601
- Full Text :
- https://doi.org/10.1093/chromsci/bmw085