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Glutathione-dependent micelles based on carboxymethyl chitosan for delivery of doxorubicin.

Authors :
Zhang, Xueqiong
Li, Chunfu
Zheng, Hua
Song, Haoyuan
Li, Lianghong
Xiong, Fuliang
Yang, Jin
Qiu, Tong
Source :
Journal of Biomaterials Science -- Polymer Edition. Dec2016, Vol. 27 Issue 18, p1824-1840. 17p. 1 Color Photograph, 1 Diagram, 9 Graphs.
Publication Year :
2016

Abstract

Novel glutathione (GSH)-dependent micelles based on carboxymethyl chitosan (CMCS) were developed for triggered intracellular release of doxorubicin (DOX). DOX-33′-Dithiobis (N-hydroxysuccinimidyl propionate)-CMCS (DOX-DSP-CMCS) prodrugs were synthesized. DOX was attached to the amino group on CMCS via disulfide bonds and drug-loaded micelles were formed by self-assembly. The micelles formed core–shell structure with CMCS and DOX as the shell and core, respectively, in aqueous media. The structure of the prodrugs was confirmed by IR and proton nuclear magnetic resonance (1H NMR) spectroscopy. The drug-loading capacity determined by UV spectrophotometry was 4.96% and the critical micelle concentration of polymer prodrugs determined by pyrene fluorescence was 0.089 mg/mL. Micelles were spherical and the mean size of the nanoparticles was 174 nm, with a narrow polydispersity index of 0.106. Moreover,in vitrodrug release experiments showed that the micelles were highly GSH-sensitive owing to the reductively degradable disulfide bonds. Cell counting kit (CCK-8) assays revealed that DOX-DSP-CMCS micelles exhibited effective cytotoxicity against HeLa cells. Moreover, confocal laser scanning microscopy (CLSM) demonstrated that DOX-DSP-CMCS micelles could efficiently deliver and release DOX in the cancer cells. In conclusion, the DOX-DSP-CMCS nanosystem is a promising drug delivery vehicle for cancer therapy. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
09205063
Volume :
27
Issue :
18
Database :
Academic Search Index
Journal :
Journal of Biomaterials Science -- Polymer Edition
Publication Type :
Academic Journal
Accession number :
118836785
Full Text :
https://doi.org/10.1080/09205063.2016.1238128