秋水仙碱对猪卵母细胞成熟及ＭⅠ期纺锤体的影响.

[Objectives] This experiment was conducted to study the dynamic changes of cytoskeleton during porcine oocyte meiotic maturation process, and the effect of colchicine on the M 1 spindle structure and in vitro maturation of porcine oocytes. [Methods] Indirect immunofluorescence and colchicine treatment wen' used to examine the cytoskeleton dynamic changes and the effect of colchicine on the MI spindle structure of porcine oocytes. [Results] The subcellular localization results showed that α-tuhulin began to assemble near the chromosome after germinal vesicle breakdown(GVBD), and formed a typical spindle structure at M I stage, then microtubules were distributed at the spindle between the two group of chromosome at AT I period, and a typical spindle Structure was formed at M 11 stage. F-actin was distributed at the cell outer periphery cortex after GVBD, and enriched at cortical region adjacent the spindle and formed aetin cap at M 1 and M 11 stage, respectively. When treated M1 stage oocytes was treated with colchicine, the meiotic spindle disappeared and the ph I extrusion rate reduced significantly. However, the spindle assembly was restored, and the ph 1 extrusion rate was also recovered after removal of colchicine. [Conclusions] The dynamic changes of the cytoskeleton were characterized by stage distribution during the maturation of porcine oocytes. Colchicine treatment resulted in the pig M 1 stage oocytes spindle dcpolymerization, and in vitro maturation inhibition, however, the effect of colchicine on the structure and function of M1 oocyte spindle had a certain degree of reversibility. [ABSTRACT FROM AUTHOR]