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Involvement of a versatile pattern recognition receptor, apolipophorin-III in prophenoloxidase activation and antibacterial defense of the Chinese oak silkworm, Antheraea pernyi.
- Source :
-
Developmental & Comparative Immunology . Dec2016, Vol. 65, p124-131. 8p. - Publication Year :
- 2016
-
Abstract
- Apolipophorin-III (apoLp-III) is an exchangeable apolipoprotein found in many insect species and functions as a lipid transport vehicle. Recent studies have shown that apoLp-III is a multifunctional molecule involved in not only lipid transportation but also innate immune responses. In the present study, the pattern recognition properties of Antheraea pernyi apoLp-III were investigated. Recombinant Ap -apoLp-III was bound to different species of microbes and further study showed the r Ap -apoLp-III is capable of interacting with pathogen associated molecular patterns (PAMPs) on the microbial cell surface. In addition, an Ap -apoLp-III/PAMP mixture stimulated the prophenoloxidase (PPO) activation of A. pernyi hemolymph in vitro , to a greater extent than PAMP alone while Ap -apoLp-III itself failed to activate the PPO system, indicating that Ap -apoLp-III up-regulates PPO activation by combining with PAMP. After pathogen invasion following an injection of Staphylococcus aureus , RNAi-mediated silencing of apoLp-III decreased the transcriptional abundance of three antimicrobial peptide genes. These data suggest that apoLp-III is a versatile pattern recognition receptor and may play important roles in the innate immune responses of Antheraea pernyi . [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 0145305X
- Volume :
- 65
- Database :
- Academic Search Index
- Journal :
- Developmental & Comparative Immunology
- Publication Type :
- Academic Journal
- Accession number :
- 118102932
- Full Text :
- https://doi.org/10.1016/j.dci.2016.07.001