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A single dual-emissive nanofluorophore test paper for highly sensitive colorimetry-based quantification of blood glucose.

Authors :
Huang, Xiaoyan
Zhou, Yujie
Liu, Cui
Zhang, Ruilong
Zhang, Liying
Du, Shuhu
Liu, Bianhua
Han, Ming-Yong
Zhang, Zhongping
Source :
Biosensors & Bioelectronics. Dec2016, Vol. 86, p530-535. 6p.
Publication Year :
2016

Abstract

Fluorescent test papers are promising for the wide applications in the assays of diagnosis, environments and foods, but unlike classical dye-absorption-based pH test paper, they are usually limited in the qualitative yes/no type of detection by fluorescent brightness, and the colorimetry-based quantification remains a challenging task. Here, we report a single dual-emissive nanofluorophore probe to achieve the consecutive color variations from blue to red for the quantification of blood glucose on its as-prepared test papers. Red quantum dots were embedded into silica nanoparticles as a stable internal standard emission, and blue carbon dots (CDs) were further covalently linked onto the surface of silica, in which the ratiometric fluorescence intensity of blue to red is controlled at 5:1. While the oxidation of glucose induced the formation of Fe 3+ ions, the blue emission of CDs was thus quenched by the electron transfer from CDs to Fe 3+ , displaying a serial of consecutive color variations from blue to red with the dosage of glucose. The high-quality test papers printed by the probe ink exhibited a dosage-sensitive allochromatic capability with the clear differentiations of ~5, 7, 9, 11 mM glucose in human serum (normal: 3–8 mM). The blood glucose determined by the test paper was almost in accordance with that measured by a standard glucometer. The method reported here opens a window to the wide applications of fluorescent test paper in biological assays. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
09565663
Volume :
86
Database :
Academic Search Index
Journal :
Biosensors & Bioelectronics
Publication Type :
Academic Journal
Accession number :
118102729
Full Text :
https://doi.org/10.1016/j.bios.2016.07.021