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Molecular epidemiology of GI and GII noroviruses in sewage: 1-year surveillance in eastern China.

Authors :
Zhou, N.
Lin, X.
Wang, S.
Tao, Z.
Xiong, P.
Wang, H.
Liu, Y.
Song, Y.
Xu, A.
Source :
Journal of Applied Microbiology. Oct2016, Vol. 121 Issue 4, p1172-1179. 8p. 2 Diagrams, 2 Charts, 2 Graphs.
Publication Year :
2016

Abstract

Aims To determine the concentration and molecular epidemiology of GI and GII noroviruses in sewage in China. Methods and Results Twenty-three raw sewage samples were collected in the cities of Jinan and Linyi, eastern China in 2014. GI and GII noroviruses were positive in all samples after TaqMan-based quantitative PCR. The mean concentrations of GI and GII noroviruses were 4·52 × 104 and 7·88 × 104 genome copies per litre respectively. After reverse transcription- PCR, cloning and sequencing, 16 genotypes were identified. GI.6 (69·6%), GI.2 (65·2%), GII.13 (65·2%), GII.6 (60·9%) and GII.17 (60·9%) were the most common GI and GII genotypes. A recombination event was observed in two GI.6 sequences. GII.4 sequences belonged to Sydney 2012 and Den Haag 2006b variant. Interestingly, the novel GII.17 Kawasaki308 variant was detected. Conclusions These results reveal that multiple norovirus genotypes cocirculated in the local population. The risk of acute gastroenteritis outbreak is high in the two cities due to the detection of GII.17 Kawasaki308 variant and the high concentration of norovirus in raw sewage. Significance and Impact of the Study This study demonstrates sewage surveillance can be a useful approach to monitor norovirus circulating in the population. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
13645072
Volume :
121
Issue :
4
Database :
Academic Search Index
Journal :
Journal of Applied Microbiology
Publication Type :
Academic Journal
Accession number :
118092713
Full Text :
https://doi.org/10.1111/jam.13218